Authors:
Giorgia Totonelli, Panagiotis Maghsoudlou, Massimo Garriboli, Johannes Riegler, Giuseppe Orlando, Alan J. Burns, Neil J. Sebire, Virpi V. Smith, Jonathan M. Fishman, Marco Ghionzoli, Mark Turmaine, Martin A. Birchall, Anthony Atala, Shay Soker, Mark F. Lythgoe, Alexander Seifalian, Agostino Pierro, Simon Eaton, & Paolo De Coppi
Summary:
Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.
Source:
Biomaterials; Vol. 33, Issue 12, 3401-3410 (04/12)