Damaged leg muscles grew stronger and showed signs of regeneration in three out of five men whose old injuries were surgically implanted with extracellular matrix (ECM) derived from pig bladder (pictured), according to a new study conducted by researchers at the University of Pittsburgh School of Medicine and the
McGowan Institute for Regenerative Medicine
. Early findings from a human trial of the process and from animal studies were published recently in Science Translational Medicine
The team was co-led by McGowan Institute for Regenerative Medicine deputy director Stephen Badylak, DVM, PhD, MD, professor of surgery at Pitt, and J. Peter Rubin, MD, UPMC professor and chair of plastic surgery, Pitt School of Medicine, professor of bioengineering, University of Pittsburgh, co-director of the Adipose Stem Cell Center, and founder and director of the UPMC Center for Innovation in Restorative Medicine, Department of Surgery, University of Pittsburgh, and also included McGowan Institute for Regenerative Medicine affiliated faculty members:
- Christopher Dearth, PhD, research assistant professor, Department of Surgery, University of Pittsburgh,
- Fabrisia Ambrosio, PhD, MPT, assistant professor, Department of Physical Medicine & Rehabilitation, University of Pittsburgh
- Michael Boninger, MD, endowed chair and professor, Department of Physical Medicine & Rehabilitation, University of Pittsburgh
- Neill Turner, PhD, research assistant professor, Department of Surgery, University of Pittsburgh
- Spencer Brown, PhD, professor of plastic surgery, Department of Plastic Surgery, University of Pittsburgh, and executive director for the UPMC Center for Innovation in Restorative Medicine, Department of Surgery, University of Pittsburgh
When a large volume of muscle is lost, typically due to trauma, the body cannot sufficiently respond to replace it, explained senior investigator Dr. Badylak. Instead, scar tissue can form that significantly impairs strength and function.
Pig bladder ECM has been used for many years as the basis for medical products for hernia repair and treatment of skin ulcers. It is the biologic scaffold that remains left behind after cells have been removed. Previous research conducted by Dr. Badylak’s team suggested that ECM also could be used to regenerate lost muscle by placing the material in the injury site where it signals the body to recruit stem and other progenitor cells to rebuild healthy tissue.
“This new study is the first to show replacement of new functional muscle tissue in humans, and we’re very excited by its potential,” Dr. Badylak said. “These are patients who can’t walk anymore, can’t get out of a car, can’t get up and down from a chair, can’t take steps without falling. Now we might have a way of helping them get better.”
For the Muscle Tendon Tissue Unit Repair and Reinforcement Reconstructive Surgery Research Study, which is sponsored by the U.S. Department of Defense and is continuing to enroll new participants, 5 men who had at least 6 months earlier lost at least 25 percent of leg muscle volume and function compared to the uninjured limb underwent a customized regimen of physical therapy for 12 to 26 weeks until their function and strength plateaued for a minimum of 2 weeks.
Then, study lead surgeon Dr. Rubin surgically implanted a “quilt” of compressed ECM sheets designed to fill into their injury sites. Within 48 hours of the operation, the participants resumed physical therapy for up to 26 additional weeks.
The researchers found that 3 of the participants, 2 of whom had thigh injuries and 1 a calf injury, were stronger by 20 percent or more 6 months after the surgery. One thigh-injured patient improved on the “single hop test” by 1,820 percent, and the other had a 352 percent improvement in a chair lift test and a 417 percent improvement in the single-leg squat test. Biopsies and scans all indicated that muscle growth had occurred. Two other participants with calf injuries did not have such dramatic results, but both improved on at least one functional measure and said they felt better.
“This work represents an important step forward in our ability to repair tissues and improve function with materials derived from natural proteins. There will be more options to help our patients,” Dr. Rubin said.
The study also showed 6 months after an injury, mice treated with ECM showed signs of new muscle growth while untreated mice appeared to form typical scars.
The project was supported by research grants from the U.S. Department of the Interior and National Institutes of Health grants AG042199 and HL76124-6.
The U.S. Department of Defense’s Limb Salvage and Regenerative Medicine Initiative and the Muscle Tendon Tissue Unit Repair and Reinforcement Reconstructive Surgery Research Study are collaboratively managed by the Office of the Secretary of Defense. The Initiative is focused on rapidly and safely transitioning advanced medical technology in commercially viable capabilities to provide wounded warriors the safest and most advanced care possible today.
Illustration: McGowan Institute of Regenerative Medicine.
UPMC/University of Pittsburgh Schools of the Health Sciences Media Relations News Release (04/30/14)
Pittsburgh Tribune-Review (04/30/14)
ABC News (04/30/14)
Associated Press (via U.S. News & World Report and Washington Post) (04/30/14)
CCTV America (04/30/14)
Pittsburgh Post-Gazette (05/01/14)
Muscle Tendon Tissue Unit Repair and Reinforcement Reconstructive Surgery Research Study
Study Information or call 412-624-5308
Bio: Dr. Stephen Badylak
Bio: Dr. Peter Rubin
Bio: Dr. Christopher Dearth
Bio: Dr. Fabrisia Ambrosio
Bio: Dr. Michael Boninger
Bio: Dr. Neill Turner
Bio: Dr. Spencer Brown
Abstract (Science Translational Medicine; 30 April 2014: Vol. 6, Issue 234, p. 234ra58.)