Researchers at Karolinska Institutet have developed the first method for directly measuring the extent to which drugs reach their targets in the cell. The method could make a significant contribution to the development of new, improved drug substances.
Most drugs operate by binding to one or more proteins and affecting their function, which creates two common bottlenecks in the development of drugs; identifying the right target proteins and designing drug molecules able to efficiently seek out and bind to them. No method has been available for directly measuring the efficiency of the drug molecules to locate and bind to its target protein. Now researchers from Karolinska Institutet have developed a new tool called CETSA (Cellular Thermal Shift Assay), which utilize the concept that target proteins usually get stabilized when drug molecules bind.
"We have shown that the method works on a wide variety of target proteins and allows us to directly measure whether the drug molecules reach their targets in cells and animal models," says lead investigator Professor Pär Nordlund (pictured) of the Department of Medical Biochemistry and Biophysics. "We believe that CETSA will eventually help to improve the efficiency of many drugs and contribute to better drug molecules and more successful treatments."
The lack of methods to directly measure the binding of a drug to its target protein has caused a degree of uncertainty in many phases of drug development. In some cases, where drug candidates have not lived up to expectations in clinical trials on humans, it has transpired that the drug molecules have failed to bind to the right protein. The group behind the study believes that CETSA will be an important control stage and a complement to other methods.
In the present study, the researchers also examined processes that can lead to drug resistance in cells. The team believes that by virtue of its ability to determine whether existing drugs are suitable for individual patients, the method is of potential value to the practice of individualized treatment.
Illustration: Karolinska Institutet.
Karolinska Institutet News Release (07/05/13)
Science Daily (07/05/13)
e! Science News (07/05/13)
Abstract (Science; Vol. 341, No. 6141, 84-87 (07/05/13))