Authors: Rapolas Zilionis, Camilla Engblom, Christina Pfirschke, Virginia Savova, David Zemmour, Hatice D. Saatcioglu, Indira Krishnan, Giorgia Maroni, Claire V. Meyerovitz, Clara M. Kerwin, Sun Choi, William G. Richards, Assunta De Rienzo, Daniel G. Tenen, Raphael Bueno, Elena Levantini, Mikael J. Pittet, Allon M. Klein
Summary: Tumor-infiltrating myeloid cells (TIMs) comprise monocytes, macrophages, dendriticcells, and neutrophils, and have emerged as key regulators of cancer growth. Thesecells can diversify into a spectrum of states, which might promote or limit tumoroutgrowth but remain poorly understood. Here, we used single-cell RNA sequencing (scRNA-seq)to map TIMs in non-small-cell lung cancer patients. We uncovered 25 TIM states, mostof which were reproducibly found across patients. To facilitate translational researchof these populations, we also profiled TIMs in mice. In comparing TIMs across species,we identified a near-complete congruence of population structures among dendriticcells and monocytes; conserved neutrophil subsets; and species differences among macrophages.By contrast, myeloid cell population structures in patients’ blood showed limitedoverlap with those of TIMs. This study determines the lung TIM landscape and setsthe stage for future investigations into the potential of TIMs as immunotherapy targets.
Source: Immunity, 2019