Authors:
Gražvydas Lukinavičius, Claudia Blaukopf, Elias Pershagen, Alberto Schena, Luc Reymond, Emmanuel Derivery, Marcos Gonzalez-Gaitan, Elisa D’Este, Stefan W. Hell, Daniel Wolfram Gerlich, & Kai Johnsson
Summary:
Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.
Source:
Nature Communications; 6, 8497 (10/01/15)