The latest version of a microfluidic device for capturing rare circulating tumor cells (CTCs) is the first designed specifically to capture clusters of two or more cells, rather than single cells. The new device called the Cluster-Chip was developed by the same Massachusetts General Hospital (MGH) research team that created previous microchip-based devices. Recent studies by MGH investigators and others have suggested that CTC clusters are significantly more likely to cause metastases than single circulating tumor cells.
“Early theories of cancer metastasis were based on clumps of tumor cells traveling through the bloodstream, but given that CTC clusters are even rarer in the blood than single CTCs, they have attracted minimal attention for several decades,” explains Mehmet Toner, PhD, director of the BioMicroElectroMechanical Systems (BioMEMS) Resource Center in the MGH Center for Engineering in Medicine, the paper's senior author. “The ability to isolate intact clusters will enable us to investigate carefully their role in the metastatic process, and understanding metastasis really is the ‘Holy Grail’ of cancer research.”
CTCs are living solid tumor cells found in the bloodstream at extremely low levels – about one in a billion cells. Starting in 2007, MGH researchers have developed three microchip-based devices that capture CTCs in ways that preserve molecular information that can help guide clinical treatment. The first two versions used antibodies directed at specific proteins on the surface of tumor cells, which limited the ability to capture cells that may have lost those marker proteins during the process of metastasis. The third version, developed in 2013, uses antigen-independent methods of isolating CTCs, which is also the case for the Cluster-Chip.
“Cancer is an extremely heterogeneous disease, and even within the same tumor you can find cells with different surface antigens,” says Fatih Sarioglu, PhD, co-lead author. “Since we are capturing clusters because of their physical properties, this chip is directly applicable to all types of cancer.” Formerly a postdoctoral fellow at the BioMEMS Resource Center, Sarioglu is now on the faculty at the Georgia Institute of Technology.
Sarioglu explains that the strategy behind the design of the chip is based on the physical properties of clusters of cells. The 3- by 1-½-inch plastic chip through which a blood sample is passed consists of rows of triangular microposts arranged in such a way that clusters passing between two posts will become trapped on the apex of a third central post and held in place by the balanced flow of fluid on either side. Single CTCs and blood cells will pass right through without being captured. In addition, passing the sample through the device at a slow rate minimizes the possibility that clusters will be broken, distorted, or escape.
Initial testing of the Cluster-Chip with blood samples to which artificially formed tumor cell clusters had been introduced helped to determine the optimal flow rate to capture the most clusters in the least time. The researchers then compared the new device to the second-generation HBCTC-Chip, which relies on known cell-surface markers and was the first to isolate CTC clusters. The Cluster-Chip proved to be 40 to 50 percent better at finding clusters of cells expressing targeted markers and 100 percent better at capturing cells without target antigens. While initial attempts to release captured clusters from the device by simply reversing the fluid flow had limited success, the investigators found that reducing the temperature of the device itself to 4 C (39 F) not only released 80 percent of clusters but also improved the purity of the captured material by reducing the undesired capture of white blood cells.
Use of the Cluster-Clip to test blood samples from 60 patients with either breast cancer, melanoma, or prostate cancer successfully captured CTC clusters in from 30 to 40 percent of samples. Analysis of captured clusters revealed they consisted of cells with significant molecular differences, some actively proliferating and other relatively quiescent, and were often accompanied by immune cells, an observation that could have important implications with the increased attention to immune-system-based cancer therapies.
“Testing of patient blood samples also revealed that there were significantly more CTC clusters in the blood than was previously believed,” says Toner. “We now are looking at ways to improve further the release of captured clusters, but we are only at the beginning of our quest to understand the role and biology of CTC clusters. Eventually we could develop ways to target clusters therapeutically as well as using them as a source of diagnostic information.” Toner is the Benedict Professor of Surgery at Harvard Medical School, and Sarioglu is an assistant professor in the School of Electrical and Computer Engineering at Georgia Tech.
“This new isolation device will be particularly useful in isolating clusters of CTCs, which seem to be the most malignant and metastasis-prone types of cancer cells in the blood,” says Daniel Haber, MD, director of the MGH Cancer Center and a co-author of the paper. “I’m particularly excited by the way in which the device was created – starting from a clinical and biological observation about the importance of these CTC clusters and then designing a specific microfluidic device to capture these cells, which will make it possible to study them in greater detail.”
Illustration: A three-cell circulating tumor cell cluster is captured and held by the balanced fluid flow on either side of the triangular microposts on the Cluster-Chip. –BioMEMS Resource Center, Massachusetts General Hospital.
Massachusetts General Hospital News Release (05/18/15)
National Institutes of Health News Release (05/18/15)
Abstract (Nature Methods; 12, 685-691 (05/18/15))