A new study showing the ability to apply a thin coating of viable respiratory epithelial cells to tissue engineered constructs using a commercially available spray device is especially promising for therapeutic approaches in development to repair or replace challenging structures such as trachea or bronchi. The effects of factors such as air pressure and nozzle diameter on cell survival and the ability of the respiratory cells to differentiate and proliferate are described in an article published recently.
In the article, Anja Lena Thiebes, Stefanie Albers, Dr. Med. Stefan Jockenhoevel, and Christian Cornelissen, Rhine-Westphalia Institute of Technology (RWTH) Aachen University, and Christian Klopsch, University of Rostock, Germany, compared the results for spray application of vascular smooth muscle cells or respiratory epithelial cells, with and without fibrin gel. Fibrin acts like a glue to hold cells and tissues together, helping to keep the cells in place until it slowly degrades over a few days, allowing the cells to differentiate.
"The work presented here is of significant importance to regenerative medicine therapeutics, providing a highly promising approach to coating tissue engineered constructs," says BioResearch Open Access Editor Jane Taylor, PhD, Medical Research Council (MRC) Centre for Regenerative Medicine, University of Edinburgh, Scotland.
Illustration: Microsoft clipart.
Mary Ann Liebert, Inc Publishers News Release (07/14/15)
Abstract (BioResearch Open Access; Vol. 4, Issue 1, 278-287 (06/01/15))