Authors:
Stephen J. Curtis, Kerstin W. Sinkevicius, Danan Li, Allison N. Lau, Rebecca R. Roach, Raffaella Zamponi, Amber E. Woolfenden, David G. Kirsch, Kwok-Kin Wong, & Carla F. Kim
Summary:
Successful cancer therapy requires the elimination or incapacitation of all tumor cells capable of regenerating a tumor. Therapeutic advances therefore necessitate the characterization of the cells that are able to propagate a tumor in vivo. We show an important link between tumor genotype and isolation of tumor-propagating cells (TPCs). Three mouse models of the most common form of human lung cancer each had TPCs with a unique cell-surface phenotype. The cell-surface marker Sca1 did not enrich for TPCs in tumors initiated with oncogenic Kras, and only Sca1-negative cells propagated EGFR mutant tumors. In contrast, Sca1-positive cells were enriched for tumor-propagating activity in Kras tumors with p53 deficiency. Primary tumors that differ in genotype at just one locus can therefore have tumor-propagating cell populations with distinct markers. Our studies show that the genotype of tumor samples must be considered in studies to identify, characterize, and target tumor-propagating cells.
Source:
Cell Stem Cell; Vol. 7, Issue 1, 127-133 (07/02/10)